Sentence examples for buffer before the antibody from inspiring English sources

In order to block nonspecific protein binding, bovine serum albumin was added to the buffer before the antibody incubation steps in the MLH1 and MSH2 stainings.

The membrane was then washed three times with Tween-TBS buffer before the HRP-conjugated secondary antibody is added at 1 µg/mL for 2 h at room temperature.

For long-term storage, the buffer of the antibody conjugate was changed to phosphate buffered saline (PBS).

The cells were washed three times in blocking buffer before secondary antibody application.

For IL-10 detection, the procedures were similar with the following exceptions: standards and sample were incubated for 3 h at RT and following this incubation, plates were rinsed 3× with wash buffer before the biotinylated anti-IL-10 antibody was added.

The membrane was then washed in TBST wash buffer before applying the secondary antibody (Anti-mouse IgG, peroxidase-linked species-specific whole antibody from sheep, GE healthcare, Piscataway, NJ) for one hour at room temperature.

The membranes were incubated overnight at 4 °C with primary antibodies against phospho-STAT3 (Tyr705), STAT3 phospho-p44/42 phospho-p44/42(Erk1/2) (Thr202/Tyr204), p44/42 MAPK (Erk1/2) (137F5), p44/42o-Akt (Ser473), and Akt and then were washed three tiMAPKwith TBST buffErk1/2ore adding the secondary antibody.

That half-children, half-adult buffer before the news".

Tissue slides were microwaved in 0.01  M sodium citrate buffer (pH 6) near boiling for 20 min, and cooled for 30 min in the buffer before incubation with the primary antibodies.

Briefly, both attached and floating cells were collected and resuspended in binding buffer before adding the Annexin V-FITC antibody and propidium iodide.

After two hours at room temperature, the plates were washed with EIA buffer before addition of the conjugate antibody (2 Ellman's units per ml EIA buffer).