Sentence examples for buffer before the analysis from inspiring English sources

Since the detection range was not within the physiological range of SL (0.1 to 2.5 mM) [ 38], the saliva sample required dilution (1 : 4) with the working buffer before the analysis.

Promastigotes were homogenized in the Western blot buffer before immunoblot analysis.

Then the electrode was washed by gentle immersion in the PBS buffer before analysis of its electrochemical properties with respect to the introduction of a redox probe electrolyte (50 μL of 10 mM [Fe CN 6]3− + 10 mM [Fe CN 6]4− was prepared in 100 mM PBS) [23].

A conducting polymer, poly 3,4-ethylenedioxythiophene) (poly 3,4-ethylenedioxythiophenemically as a thin layer on a glassy carbon electrode and then acclimatized to an aqueous buffer before samPEDOTnalysis.

The precipitate was washed 4 times with CHAPS buffer and boiled 5 min in loading buffer before immunoblot analysis.

Immunocomplex pellets were washed extensively and boiled in protein sample buffer before immunoblot analysis.

The beads were then washed with RIPA buffer for three times and resuspended in 1X SDS sample buffer before Western analysis.

Immunoprecipitates were washed four times in the lysis buffer before Western blotting analysis.

To analyze surface expression of HLA-I Ag, cells were washed and resuspended in FACS buffer (phosphate- buffered saline pH 7.2, 0.2% bovine serum albumin, and 0.02% sodium azide), then incubated with phycoerythrin (PE -conjugated-HLA-I (Beckman Coulter) for 15 minutes at 4°C, and finally washed with the same buffer before flow cytometry analysis.

For subsequent antiphosphotyrosine immunoblot analysis, precipitates were washed three times with ice-cold PBS, resuspended in 50  μl of 2 × Laemmli buffer, and boiled for 5 min at 100°C before the analysis.

The incubation was terminated by 10-fold dilution with ice-cold PBS buffer before the cells were washed and submitted to FACS analysis.