Sentence examples for buffer before the addition from inspiring English sources

Immunoprecipitates were washed twice with RIPA buffer before the addition of 2× Laemmli buffer.

Compounds (133 × final concentration in DMSO) were diluted into Refolding buffer before the addition of NS2 3 to yield required final concentrations.

Plates were then washed 7 times in Delfia™ wash buffer before the addition of 100 µL·well−1 of enhancement solution (PerkinElmer).

Briefly, sections were post-fixed and equilibrated in terminal transferase buffer before the addition of reaction buffer containing digoxigenin-dNTP oligonucleotide.

The immunocomplexes recovered on beads were washed two times for 5 min with 1 ml of RIPA buffer before the addition of Laemmli sample buffer.

Neutravidin agarose resin (375 μL slurry volume) was washed twice with lysis buffer before the addition of diluted lysates containing the affinity capture agent.

Briefly, caspases were incubated for 5 min in the reaction buffer at 37 °C before the addition of 5  μl of translation product to the final volume of 25  μl.

Pellets were resuspended in hypotonic buffer for 10 min before the addition of sample buffer, whilst sample buffer was added directly to all supernatant fractions.

To detect intracellular TTP, fixed cells were washed twice in permeabilization buffer (consisting of staining buffer with the addition of 1 μg/ml saponin), centrifuged as before, and incubated for 30 min at 4°C in blocking buffer, consisting of 3% IgG with 3% nonfat powdered milk for leukocytes in permeabilization buffer.

Following washing cells were again incubated in blocking buffer for 30 minutes, before the addition of the secondary antibody (goat anti-mouse Alexa fluor 488, cat# A11001, Invitrogen) diluted 1∶500 in blocking buffer for 1 hour at 37°C.

After two hours at room temperature, the plates were washed with EIA buffer before addition of the conjugate antibody (2 Ellman's units per ml EIA buffer).