Sentence examples for buffer before quantification of from inspiring English sources

Beads were extensively washed with binding buffer before quantification of bounded peptide by Cerenkov counting.

Doublets and debris were gates out before quantification of median fluorescent intensities with FlowJo (FlowJo, LLC).

Cells were left for 2 days following transfection, before quantification of AKT1 phosphorylation or PS staining.

Cells were subsequently washed three times in wash buffer and resuspended in cold PBS containing 2% (w/v) FBS before immediate quantification of ligand internalisation using a FacsCalibur flow cytometer (Becton Dickinson).

Therefore, it is often necessary to store surface water samples before the quantification of pharmaceutical products, pesticides and steroidal hormones.

After washes, infection was allowed to proceed for 16 hours before analysis quantification of CHIKV-positive cells by flow cytometry.

Immunoprecipitates were washed twice with RIPA buffer before the addition of 2× Laemmli buffer.

Manual dilution was performed before quantification in the case of the highest concentrations.

Following transfection, neurons are cultured additional 1, 3 or 5 days before imaging and quantification of total dendritic length.

Transfected cells were exposed to androgens for 48 hr before harvesting and quantification of luciferase activity.

A pure mitochondria layer was collected below the peroxisome layer and washed twice in mitochondrial isolation buffer before being submitted to protein quantification.