Sentence examples for buffer before determination of from inspiring English sources

Protein lysates from cell lines were prepared using previously published protocols (Gilmour et al, 2002); the same method was used for primary tumour samples, but in these cases tissue was initially homogenised in extraction buffer before determination of protein content.

The trichloroacetic acid was extracted with acetone and the extract neutralized in phosphate-HEPES buffer before determination of cAMP by a competitive binding assay based on displacement of [H]cAMP from the RI α subunit of PKA.

Before determination of scene transitions, the histogram of point distance of each frame is calculated.

All strains were pre-grown on glucose before determination of μmax on pentose sugars.

Cells were incubated for an additional 48 hours before determination of cell viability.

The specimen was freeze-dried for 48 hr before determination of the dry weight.

Baselines were subjected to a first-order drift correction before determination of peak areas.

MglA proteins were made nucleotide free before determination of nucleotide affinities (Supplementary data).

Before determination of suitable features, an image preprocessing has to be performed.

We used six volumes of lysis buffer for determination of IFN-α in liver samples and eight volumes of buffer for determination of IFN-γ.

After precipitation and a second centrifugation, the samples were dried under a nitrogen flow and resuspended in 300 μl of assay buffer before RIA determination.