Sentence examples for buffer before assay of from inspiring English sources
In the case of DTT, excess reagent (which interferes with the formation of TNB from DTNB) was removed by centrifugation at 11300 g for 2 min using a 10 kDa molecular-mass cut-off filter and subsequent washing with two aliquots of buffer before assay of the enzyme activity.
Well-fed J4 and adult worms were washed in M9 buffer twice before assay.
Tissues were homogenized in phosphatase inhibitor containing solubilization buffer, before protein assay and immunoblot analysis.
After the last step, a 3′-phosphoadenosine 5′-phosphate (PAP -agarose affinity column, we dialyzed the fractions eluted with PAP -agaroseee chaffinity buffer to remove PAP before the assay of SULT acolumny weth 3-OH-BaP as substrate.
The trichloroacetic acid was extracted with acetone and the extract neutralized in phosphate-HEPES buffer before determination of cAMP by a competitive binding assay based on displacement of [H]cAMP from the RI α subunit of PKA.
Immunoprecipitates were washed twice with RIPA buffer before the addition of 2× Laemmli buffer.
The proteoliposomes were on ice and resuspended in cold assay buffer before use with a final concentration of 100 mg/mL.
After precipitation and a second centrifugation, the samples were dried under a nitrogen flow and resuspended in 300 μl of assay buffer before RIA determination.
Lipid extracts were evaporated under a stream of nitrogen and reconstituted in assay buffer before 15s-HETE and 9s-HODE determination by enzyme-linked immunosorbent assays (ELISAs; 15(S -HETE ELIS -HETE ELISALife Sciences, Farmingdale, NY, USA; 9-HODE EIA Kit, Oxford BiomEnzo Life Sciencesochester Hills, MI, USA).
GPx (1.5 μM protein) was incubated at room temperature (22°C) for 120 min in the absence (buffer added) or presence of added HOSCN (10 100 μM) in 100 mM phosphate buffer (pH 7.4), before assaying enzyme activity.
