Sentence examples for buffer before addition of from inspiring English sources

After two hours at room temperature, the plates were washed with EIA buffer before addition of the conjugate antibody (2 Ellman's units per ml EIA buffer).

After a brief spin at 10,000× g at 4°C and removal of the supernatant, the beads were washed 4 times with bead buffer before addition of 35 µl of 2× Laemmli sample buffer.

After a brief spin at 10,000 g and removal of the supernatant, the beads were washed 4 times with bead buffer before addition of 60 µl 2X Laemmli sample buffer.

The samples were aspirated, and the wells were washed five times with washing buffer before addition of rabbit anti-Rad51 antibody (Calbiochem, San Diego, CA) diluted 1∶500 in blocking buffer and incubated at 30°C for 1 h.

The nuclei were resuspended in 250 µl of NI buffer before addition of an equal volume of (10 mM HEPES [pH 7.9], 600 mM NaCl, 10% sucrose, 5 mM MgCl2, 0.1 mM EDTA, 0.5% NP-40, 1 mM DTT and protease inhibitor cocktail).

Complexes were centrifuged at 14, 000 × g and washed 5 times in wash buffer before addition of 20uL 2× Laemmli buffer and then boiled for 5 minutes.

Enzyme was pre-incubated for 20 min at 37°C in the appropriate buffers before addition of Ac-LRSR-AFC at 100 μM and determination of the initial velocity.

ATP or MgATP were prepared in same buffer before addition to protein samples.

Membranes were prehybridized for 1 hour in ULTRAhyb buffer (Ambion) before addition of radiolabelled probes.

In experiments with inhibitor, 0.5 μL inhibitor, 5 μL NF-D1, and 4.5 μL protein in binding buffer were incubated before addition of 40 μL NF-D2.

For competition assays, 25- and 100-fold excess cold competitor oligonucleotide duplex was added to the reaction buffer 10 minutes before addition of the labeled probes.