Sentence examples for buffer at the indicated from inspiring English sources
Proteins were added to this buffer at the indicated concentrations.
The kinetic constants in the pH range 7 11 were measured as described above, with the exception that the reactions were carried out in sodium phosphate buffer at the indicated pH values (5.0, 6.0, 7.0, 8.0, 9.0 and 10.0).
The reaction mixture were consist of 0.5 ml enzyme solution and 0.5 ml of 1% colloidal chitosan in 1 ml McIlvaine buffer at the indicated pH.
Microscopy was performed on cells grown in YNB medium (0.67% yeast nitrogen base without amino acids, 2% dextrose and necessary auxotrophic amino acids) plus 50 mM MES to buffer at the indicated pHs.
Samples were mixed in the same buffer at the indicated concentrations (16.5 nM tetrameric hKif15, 33 nM dimeric hTpx2) and incubated for 20 min at room temperature in the presence of 2 mM ATP or AMP-PNP.
The supernatants were collected, incubated at 30 °C for 5 min, laid on top of a sucrose cushion [containing 6% (w/v) sucrose and 0.06% Triton X-114 in the above buffer, at the indicated pH], and centrifuged at 830 g for 3 min at room temperature.
Reactions were terminated by adding SDS sample buffer at the time indicated.
For pH optimum determination, substrate was dissolved in 50 mM Tris-acetate buffer containing 150 mM NaCl at the indicated pH values.
Confluent MDCK cells were infected with virus at moi 2 in MES buffer (0.25 µg/ml Amph B) at the indicated pH values or mock-infected.
Red blood cells were lysed in NH4Cl buffer; subsequently, the cell pellets were recovered and infected with OBP-401 at the indicated concentrations.
