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Briefly, predetermined amount of Dox-loaded NPs were added into distilled PBS buffer at the different volume to obtain a homogenous suspension.
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Borate buffers at all the different pHs studied had delayed buffer exhaustion of 100 minutes, a property making borate buffers appropriate for extended electrophoretic runs.
In the case where the "costs" of placing a buffer at different locations are different, we can guarantee to insert maximum number of buffers with minimum total cost.
To use Tris-HCl buffer at different temperatures, the pH was adjusted at 25°C so that the final pH would be 7.5 at the desired temperature [ 64].
When the shoots reached a length of approximately 10 cm, they were artificially inoculated by needle prick with 10 μL suspension (10 cells mL-1) of X. fastidiosa strain 9a5c in PBS buffer at five different points on the same stem.
The anti-CD20 IgG1 was diluted in the HBS-EP buffer at six different concentrations (ranging from 4.17 to 133.3 nM).
The optimal temperature was determined in PL buffer at different temperatures in the range of 35 70°C.
Each user i is assigned a buffer at the eNodeB, and packets of different traffic classes are streamed into the buffer of the eNodeB.
Briefly, aliquots of 50 μl PRP were incubated in Krebs phosphate buffer at six different concentrations of [3H]-5-HT/5-HT ranging from 0.1 to 1.6 μM, at 37°C and 4°C for 5 min.
Thermal stability of the purified AS-protease was estimated by incubating the enzyme in 50 mM Tris buffer at different temperatures (35°C, 45°C and 55°C) in the presence of 5 mM CoCl2.
The optimal pH for each enzyme was determined by incubating each xylanase with the substrate dissolved in an appropriate buffer at different pH values: 50 mM citrate buffer (pH 2.0 6.0), 50 mM PBS buffer (phosphate-buffered saline, pH 6.0 8.0), and 50 mM glycine NaOH buffer (pH 8.0 11.0), at the optimal temperature for 10 min.
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