Sentence examples for buffer at a volume from inspiring English sources

The bacterial cells were then harvested, washed three times in an equal volume of cold wash/electroporation buffer, and suspended in the same buffer at a volume of 100-fold concentrated.

In brief, larvae were collected in RIPA buffer at a volume of 2 μl/larva, homogenized by sonication, and pelleted by centrifugation.

After washing with PBS, sections were incubated with terminal deoxynucleotidyl transferase solution in reaction buffer at a volume ratio of 9 1 at 37°C for 1 hour followed by extensive washing.

Serum samples diluted 1 100 in dilution buffer at a volume of 100 μl/well were incubated for 30 min. After washing three times with washing buffer, anti-human IgG conjugate was added to the wells (100 μl/well) and incubated for 30 min. Surplus conjugate was removed by three washing cycles.

Protein samples were mixed with 2x sample buffer at a 1∶1 volume ratio and heated at 100°C for 5 min. Samples were separated by electrophoresis on either 15% SDS-PAGE gels (microdissected lens fractions, whole lens proteins, and HLE-B3 protein extracts) or 16% Tricine-SDS-PAGE gels for CNBr cleaved proteins at room temperature for 1.5 h at 120 volts.

The samples were centrifuged in a Beckman Optima TLX ultracentrifuge at 900,000 rpm for 20 min. The pellets were dissolved by protein gel loading buffer at an equal volume as the supernatant.

Biotinylated anti-MRP8/14 (Abcam, Cambridge, UK), diluted 1/2000 in sample buffer, were added at a volume of 100 μl/well, and incubated at 4°C over night.

At the end of the indicated incubation period, the remaining medium was removed, cells were briefly washed with phosphate buffered saline (PBS) and incubated in lysis buffer (PBS/0.5% Igepal) at a volume corresponding to the volume of the tissue culture supernatant.

Female plasma samples, nestling plasma samples and the chloroform extracted yolk fraction were diluted 1∶1,000 in sample buffer and added, in duplicate, at a volume of 100 µL/well.

Ground tissue was added to an extraction buffer of CaCl2 (2 % w/v) at a volume of 2 ml for each gram of tissue, and stirred for 3 h at room temperature.

For oligonucleotide annealing, 1 nm of each forward and reverse oligonucleotide (supplementary file 1) were mixed with 1× T4 polynucleotide buffer at final volume 50 μl.