Sentence examples for buffer at a ratio from inspiring English sources

Samples for western blotting were prepared by adding Laemmli buffer at a ratio of 1 1 and heating at 95 °C for 5 min.

For LDH release assay, cell culture medium was mixed with reaction buffer at a ratio of 1 1 and the absorbance at 340 nm was measured as above.

Laemmli sample buffer at a ratio of 1 : 2 was mixed to the sample.

Young, succulent leaf tissue is macerated in cold neutral 0.05M potassium phosphate buffer at a ratio of 0.1 g of tissue to 1 ml of buffer.

For Ni-NTA pulldown experiments, each fraction was combined with pulldown buffer at a ratio of 1 1.

Working staining solution was prepared from the JC-1 powdered dye and assay buffer at a ratio of 1 99, which was then added to the cells.

Samples were ground with extraction buffer, added at a ratio of 25 : 1 for leaves and 62.5 : 1 for lyophilised tissue and consisting of PBS at pH 7.4, 10 mM Na2SO3, 2% (w/v) PVP40000, and 0.2% (w/v) BSA and supplemented with 0.05% (v/v) Triton X-100.

NIT68A was dissolved in 20 mM Tris-Cl, 150 mM NaCl, pH 7.4 at 24 mg/ml, and was mixed with PGT128 Fab (29 mg ml−1, in the same buffer) at a 1 10 ratio of PGT128: NIT68A, with a final concentration of 19.4 mg ml−1 PGT128 and 4 mM glycoside.

Additional dilution in the potassium phosphate buffer at a 1 9 ratio was applied to the liver supernatant.

Fr IIb was then loaded onto a phosphocellulose column equilibrated with dialysis buffer at a packing ratio of 0.5 mg of total protein per mL of resin.

The conditioned media samples were centrifuged at 14,000 rpm for 15 minutes at 4°C to remove contaminants and were boiled in sample buffer at a 4 1 ratio for 5 minutes.