Sentence examples for buffer at a range from inspiring English sources

For determination of optimal pH at room temperature 100 mM Tris HCl buffer at a range of 6 10 was applied, whereas the range 4 6 was measured in 10 mM acetic acid/acetate buffer.

More than 40 combinations of buffer, co-factors and antioxidant agents were tested, including TRIS vs. phosphate buffer at a range of pH values, sucrose, glucose, glycerol, detergents (CHAPS, TRITION), and Complete®.

The 5-HT3A expressing cells were treated with palonosetron (1 nM, 4°C, 150 min) and washed (on ice) in binding buffer at a range of pH values (pH 7.4, 6.5, 6.0, 5.5 or 5.0), then incubated for 10 min (on ice) in buffer at each pH.

The pH stability was determined by diluting the DyP-type peroxidase in sodium acetate buffer at a pH range of 3.0 6.0 (0.5 steps).

The pH optimum of purified recombinant xylanase was determined under the standard assay conditions, except 5.0 mg mL−1 birchwood xylan in 50 mM Na2HPO4 citric acid buffer at a pH range of 3.0 7.5.

Samples of Cel, LS, and LCCs were prepared at 50 mM sodium acetic buffer at a wide range of pH values, and 25°C for size and zeta potential measurements by DLS analyzer equipped with a laser Doppler microelectrophoresis (Zetasizer Nano ZS90, Malvern Instruments, Malvern, UK).

The purified biotinylated UvrD was immobilised on a streptavidin-coated chip, and then buffer containing UvrB at a range of concentrations was allowed to flow over the UvrD-modified surface.

To investigate whether nucleosomes could act as cofactors in the binding of these antibodies to dsDNA, the same ELISA tests were carried out on antibody diluted to a concentration of 50 μg/ml in SEC buffer containing nucleosomes at a range of concentrations from 1.5 μg DNA/ml to 20 μg DNA/ml (nucleosomes were prepared and quantified in terms of DNA content as described below).

Samples of native CtxB5 at 344 µM concentration were acidified in 0.1 M HCl/KCl buffer at pH 1.0 for 15 min and added directly in the cuvette containing McIlVaine buffers at a pH range 8.0 5.0.

Strips were then placed in 50 mL Falcon tubes and submerged in 20°C 25°C harvest buffer at a buffer to biomass ratio ranging from 10 to 25 mL/g FW.

Recombinant rM179 amelogenin was dissolved in a pH 7.4 Tris HCl buffer at concentrations ranging from 12.5 to 300 μg/ml.