Sentence examples for buffer at a flow from inspiring English sources

Forty μL of sample was injected onto a reverse phase peptide C18 Captrap (Bruker) for pre-concentration and desalted for 5 minutes with the loading buffer at a flow rate of 10 μL per minute.

The proteins were eluted with the same buffer at a flow rate of 1 mL min-1.

The mobile phase was 39 % MeOH in 0.1 M NH4HCO2 buffer at a flow rate of 5 mL/min.

Before using the capillary for continuous hydrolysis, it was rinsed and flushed with 5 mL of protein buffer at a flow rate of 400 µL/min.

Fifty mM sodium phosphate buffer (pH 7.0) was used as the elution buffer at a flow rate of 0.5 mL/min.

The dialyzed fraction was concentrated to 1 ml in sucrose and loaded on a Biogel P-150 column (45 × 0.8 cm) equilibrated with phosphate buffer (pH 7.8) and eluted with same buffer at a flow rate of 3 ml/h.

Figure 3 Effect of pH of carrier stream on the reaction efficiency of CTH packed reactor using 0.04 M phosphate buffer at a flow rate of 0.25 mL/min and packed reactor of temperature 40°C.

The column was equilibrated with phosphate buffer (pH 6.5) for 24 h and eluted with 0 1.0 M linear gradient of NaCl in 50 mM Na-malonate buffer at a flow rate of 0.5 mL/min.

The influence of this parameter on the analytical signal was studied in the pH range of 2.8-5 using 0.04 M phosphate buffer at a flow rate of 0.25 mL/min and packed reactor temperature of 40°C.

The column was rinsed thoroughly with the same buffer and then eluted with 100 mM methyl-α-d-mannoside in the buffer at a flow rate of 20 ml/h.

The unbound fraction (the PS-I fraction) was rinsed and washed with the same buffer, and eluted with the same buffer containing 100 mM methyl-α-d-mannoside in the buffer at a flow rate of 20 ml/h (Fig. 1b).