Sentence examples for buffer at a final from inspiring English sources
The cells were then again re-dispersed in 50 mM potassium phosphate buffer at a final cell O.D. of 0.5 at 600 nm.
For transformation, the protoplasts were centrifuged for 5 min at 3,000 × g, washed several times with transformation buffer [1M sorbitol, 80 mM CaCl2, pH 7.0 (Walz and K°Ck, [1993])] and re-suspended in the same buffer at a final concentration of 5 × 107.ml-1 107.ml-1
All probes were added to hybridization buffer at a final concentration of 5 ng/µl.
After a washing step, cells were suspended in load buffer at a final concentration of 2×106 cells/mL and stored at 4°C.
To culture NB-like particles from boiled protein solutions as shown in Fig. 7, solutions of BSF purified from FBS (Sigma) or BSA purified from FBS (Sigma) were prepared in HEPES buffer at a final concentration of 25 mg/ml.
Reactions were carried out in 20 µL volumes containing 4 µL Master SYBR Green PLUS buffer at a final concentration of 1X, 5 µL of 0.1 µg/µL cDNA.
The upper, particulate diffuse cell wall enzymatically active (P60) band was collected and washed three times with buffer A and re-suspended in buffer A at a final protein concentration of 10 mg/ml.
DNA was re-dissolved in TE buffer (1×) at a final concentration of 5 ng/μl.
The first PCR reaction was performed in a total volume of 50 μl and contained 5 μl genomic DNA at a concentration of 80-100 μg/ml, 5 μl 10 fold Taq polymerase buffer, MgCl2 at a final concentration of 50 mM, 20 pmol of each primer, 4 μl of the dNTP mix (2 mM total) and 1.5 units of Taq DNA polymerase (Invitrogen, USA).
For assaying the effects of phagocytosis on gene expression, axenically growing AX2 cells in exponential phase (3 4 × 106 per ml) were washed twice in Soerensen phosphate buffer and resuspended at a final concentration of 5 × 106 per ml either in axenic medium or in Soerensen phosphate buffer containing a 1000-fold excess of E. coli B/r.
CpG were dissolved in Tris-EDTA buffer and used at a final concentration of 3 µM.
