Sentence examples for buffer at a density from inspiring English sources

Cells were washed twice in ice-cold PBS buffer and centrifuged at 900 rpm for 3 min. The pellets were resuspended in binding buffer at a density of 106 cells/mL.

Cells were resuspended in electroporation buffer at a density of 1 × 10 ml−10

For the analysis of CD44v6, cells were resuspended in FACS buffer at a density of 200000 cells/50 μl.

Cell pellets were washed with phosphate buffer twice and resuspended in 250 μL binding buffer at a density of 1 × 106 cells/mL.

Development was done with cells starved in Soerensen phosphate buffer at a density of 1×10 cells/ml in shaking suspension.

Cells were counted and preadapted in PBS buffer at a density of 2.5 x 10 cells/ml (corresponding to two cells per leukocyte) incubating 30 min at 37°C before inoculation in human blood [ 22].

We prepared VEG and STA cells used for our measurements as follows: VEG cells were suspended in 10 ml phosphate buffer (PB) at a density of 103 cells/ml, and 1.5 ml of the suspension was placed onto a 1.5% agar plate (66 mm diameter, Falcon).

PBMCs were isolated by density gradient centrifugation over Ficoll-Paque (GE Healthcare, Little Chalfont, Buckinghamshire, U.K ., washed twice in PBS-EDTA, washed once in PBS, and resuspended in Automacs Running Buffer (Miltenyi) at a density of 10 cells/mL.

The cells were scraped into a buffer containing 25 mM Tris/HCl, pH 7.5, 0.1 mM DTT (dithiothreitol), 0.1 mM EDTA, 0.1 mM EGTA, 1 μM pepstatin A, 1 μM leupeptin, 1 μM antipain and 10% (v/v) glycerol (Buffer A) at a density of ~1×10 cells/ml for a citrulline formation assay.

After passing through a 40 μm cell strainer, cells were resuspended in flow cytometry buffer at a final density of 2 × 10 cells ml−1.

For the measurement of base excision repair capacity the final cell pellet was resuspended in extraction buffer at a cell density of 10/100  μl, snap frozen in liquid nitrogen and stored at −80°C.