Sentence examples for buffer as a previous from inspiring English sources

Additionally, we incorporated higher polyethylene glycol PEG-80000) concentrations (12.5% or 15% vs 5%) in the ligation reaction buffer, as a previous report showed improved ligation efficiency under these conditions [ 18].

Cells were lysed with kinase lysis buffer as previous described [9] and western blotting was performed to evaluate the phosphorylation of AMPK.

The beads were then washed sequentially with: a) Low-salt buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8.1, 150 mM NaCl); b) High-salt buffer (as previous but with 500 mM NaCl); c) LiCl buffer (0.25 M LiCl, 1% NP-40, 1% deoxycholic acid, 1mM EDTA, 10 mM Tris-HCl pH 8.1) and d) TE buffer (1 mM EDTA, 10 mM Tris-HCl pH 8.1).

Protein extraction buffer (Buffer) served as a negative control.

The OR operation is since some receivers might have buffered the packet in a previous transmission.

Mycelium was harvested and disrupted as described in a previous section and 1 g (wet weight) was resuspended in 10 ml 20 mM Tris/HCl buffer pH 7.5.

Suppose a buffer of 50 previous frames is used to build a background model.

As mentioned above, in a previous paper [9], we have provided a detailed explanation for the behavior of Ge QDs penetrating Si3N4 buffer layers.

The buffer acts as a sand reservoir and delays marine driven cliff erosion, resulting in lower estimated cliff retreat compared to previous models neglecting protective beaches.

Wladimir acted as a buffer as Chisora continued to goad them.

Median filtering sets each color channel of a pixel in the background as modeled by the median value, obtained from a buffer of previous frames.