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Each green square corresponds to one compound and each red square corresponds to running buffer as a negative control.
Luminescence signals were measured about every 6 min for 90 min before and after adding gibberellin (GA3, 1, 10, 100 μM) or W5 buffer (as a negative control) in rice protoplasts.
Luminescence signals were measured about every 6 min for 120 min before and after adding gibberellin 3 (gibberellinrellin 4 (Gabscisiccisic acid (ABA), indoleacetic acid (IAA), kinetin (KT), salicylic acid (SA), and jasmonic acid (JA) at 10 μM and W5 buffer (as a negative control) in different wells.
An initial assessment of the IYDs probiotic viability enhancing property at a concentration of 3 g/L was performed in parallel with non-supplemented pH 3.0 buffer as a negative control and in the presence of viable S. cerevisiae EC-1118 cells as a positive control.
Primary antibodies were replaced with PBS buffer as a negative control.
For testing growth in liquid cultures, inocula of S. cerevisiae were added to liquid minimal media supplemented with one of the statins or buffer as a negative control.
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Protein extraction buffer (Buffer) served as a negative control.
The collagenase Clostridium (0.025 U/ml) was run as a positive control and running buffer acted as a negative control.
Cells were allowed to adhere overnight and then sensitized with 1 μg/well anti-PSA IgE in assay buffer (5 mM KCl, 125 mM NaCl, 20 mM Hepes, 1.5 mM CaCl2, 1.5 mM MgCl2; pH 7.4) or in assay buffer alone as a negative control.
We also used phosphate buffered saline as a negative control.
PBS buffer was used as a negative control.
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