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The mixture was cooled and the absorbance was measured at 532 nm against the buffer (as a blank control).
They were then diluted with metal-free 1 M nitric acid and analyzed along with a sample of the dialysis buffer as a blank, as well as standard copper and zinc solutions.
The withdrawn samples were analyzed by UV spectrophotometer at 225 nm using a buffer as a blank.
The protein concentration was measured using the NanoDrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA) at an absorbance of 280 nm, using lysis buffer as a blank control.
After an overnight incubation at 37°C, the OD at 590 nm were measured using a 96-well multiscanner autoreader (Dynatech MR 5000, Chantilly, VA), with the extraction buffer as a blank and reduction in viability as per treatment was calculated by comparing with untreated cells as control.
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After 2-h incubation at 37°C, the optical density (OD) at 450 nm was measured using a 96-well multiscanner autoreader with the extraction buffer used as a blank.
Wells were loaded with the diluted serums or with buffer C as a blank sample, and the plate was incubated for 1 h at 37°C for the reaction of antibody capture.
The concentration and purity of RNA were measured on a NanoDrop ND-1000 Spectrophotometer (Agilent Technologies Inc., Santa Clara, CA, USA) while using buffer (10 mM Tris HCl buffer, pH 7.5) as a blank.
Meanwhile, in the fluorescent complex, DPA supernatants were replaced by isometric Tris HCl buffer to serve as a blank control.
Buffer E served as a blank and untreated RNA (2 ml) served as a positive control.
To the fourth well containing plasma, 175 μL phosphate buffer was added as a blank.
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