Sentence examples for buffer and were washed from inspiring English sources

Then, 5×105 cells were incubated with crude cell extract containing E2 proteins or control cell extract for 1 h at room temperature in washing buffer and were washed twice with PBS.

Animals were washed from plates using M9 buffer and were washed using centrifugation.

After washing with TBST buffer, the membranes were re-blocked with 3 % BSA/TBST buffer for 1 h and were washed three times with TBST buffer.

In micro tube, it was diluted to 1 μM solutions with Trizma buffer solution and was washed with Tris-EDTA buffer solution (pH 8).

All hybridizations were carried out in SDS-based buffer, and slides were washed and dried following each hybridization as recommended (Genisphere).

For immunoprecipitation, lysates were pre-cleared with mouse anti-IgG (Zymed, Carlsbad, CA, USA) immunoprecipitations were carried out in the same buffer, and lysates were washed four times with the same buffer containing 0.1% Nonidet P-40.

To ensure that the tyrosine phosphorylation observed was on IKKβ and not autophosphorylation of FGFR4 (Figure 2A), cells were lysed in RIPA buffer, and immunoprecipitations were washed over 10% sucrose to eliminate protein-protein interactions.

Erythrocytes were lysed with ice-cold ammonium chloride buffer, and neutrophils were washed in Hanks balanced salt solution without Ca2+/Mg2+ (HBSS-/; Chemical Reagents, Beijing, China).

Erythrocytes were lysed with ice-cold ammonium chloride buffer, and neutrophils were washed in Hank's balanced salt solution (HBSS) without Ca2+/Mg 2+ (HBSS-/; Chemical reagents, Beijing, China).

The beads were suspended in IP buffer and centrifuged at 1000g for 1 min. After equilibration with IP buffer beads were washed and suspended in 50µL IP buffer.

After stopping digestion with a serum- and calcium-containing buffer, cells were washed with PBS and collected by centrifugation (180 rcf, 1 min).