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The granules were resuspended in 100 µl of HEPES buffer and were used for the various microscopy and spectroscopy analyses.
Sections were encircled with rubber cement to enclose hybridization buffer and were used immediately in hybridization.
All cell extracts were adjusted to the same protein concentration by adding lysis buffer and were used for enzyme assays.
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Specific control groups included (a) protein only, (b) peptide only, and (c) buffer only, and were used for background correction.
The pellet was washed several times with digitonin-free sucrose buffer and was used as the organelle fraction which was solubilized in RIPA buffer.
The C232S substitution obviates the need for reducing agent in the assay buffer and was used as the control for in vitro experiments (ATC232S).
The binding buffer and conditions were used as in [ 17], but 0.1 mg/ml buffer of heterologous herring sperma DNA was added.
All reagents, including the buffer and column, were used as suggested by Pickering Laboratories.
Untreated SIgA in buffer and buffer alone were used as positive and negative controls, respectively, for inhibition of biofilm formation by MO10.
Standard buffers and conditions were used for electrophoresis and immunoblotting.
Native sample buffer and native running buffer were used for nondenaturing electrophoresis.
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