Sentence examples for buffer and were subjected from inspiring English sources

Finally, proteins were extracted from the beads with SDS sample buffer and were subjected to SDS acrylamide gel electrophoresis.

Cell pellets were resuspended in RIPA buffer and were subjected to sonification.

Immunoprecipitates were washed three times in lysis buffer and were subjected to western blot for detection of rpS6.

Beads were washed and boiled with SDS gel loading buffer and were subjected to western blot using anti HIPPI and anti P53 antibodies.

After incubation for 2 hours at 4°C, the beads were washed three times with the lysis buffer, and the immunoprecipitates were eluted using 3× Laemmli sample buffer and were subjected to SDS-PAGE and western blotting using indicated antibodies.

Immunoprecipitates were collected on Protein G agarose beads by overnight rotation, washed four times with lysis buffer, re-suspended in 2× Laemmli sample buffer, and were subjected to SDS-PAGE followed by Western blot analysis.

Labelled CD44 was released from the beads via incubation (100°C/5 minutes) in 2× reducing Lamelli sample buffer, and was subjected to SDS-PAGE and immunoblotting.

Samples were centrifuged (13 000 rpm for 5 min) and the lysate collected; pelleted material was resuspended in a second aliquot of lysis buffer and was subjected to the 15 min of vortexing followed by centrifugation.

The spore pellets were washed twice with the spore binding buffer as described above, and were subjected to PLG binding assays.

Sequencing reactions were performed in a total volume of 7.5 μl, each reaction contained 3.2 μM primer, BigDye® Terminator v3.1 (Life Technologies Australia Pty Ltd, Victoria, Australia), BigDye® sequencing buffer (Life Technologies Australia Pty Ltd, Victoria, Australia) and were subjected to cycling conditions as described in the BigDye® v.3.1 protocol.

Supernatants were added with SDS sample buffer (pH 6.8) containing 8 M urea and were subjected to 12.5% SDS/PAGE.