Sentence examples for buffer and were stained from inspiring English sources

Cells were washed, fixed and permeabilized in FACS permeabilization buffer and were stained by a panel of conjugated antibodies (fluorescein isothiocyanate (FITC), phycoerythrin (PE), peridinin chlorophyll protein (PerCP) and allophycocyanin (APC) including antibodies to human CD4, CD8, CD69 (Pharmingen), and IFN-γ and respective isotype controls.

Cells were washed gently three times with warm buffer and were stained with counterstains as desired amount for imaging.

The reaction products were separated on 1.3% agarose gels in TBE buffer and were stained using ethidium bromide.

The slides were washed thoroughly with neutralization buffer and were stained with 10  μL of ethidium bromide (10  μg/mL) and viewed under confocal laser scanning microscopy.

After adding 300  μl of FACS buffer and centrifuged at 5000 × g for 5 min, cell pellets were resuspended with 700  μl of FACS buffer and were stained using 0.25  μg/ml 7-amino-actinomycin (Sigma, St . Louis MO, USA) for 10 min at room temperature.

The cell pellets were re-suspended in citrate buffer and nuclei were stained with propidium iodide (Sigma-Aldrich, Bornem, Belgium) as described previously (Vindelov et al, 1983).

Following first and secondary antibody incubations in the same buffer, nuclei were stained with blue DAPI (1 μg/ml, Sigma Aldrich).

Briefly, after performing the indicated treatments, cells were harvested and washed twice with pre-cold PBS buffer, and then cells were stained with Annexin V and PI in 1 × binding buffer.

After incubation with the blocking buffer, cells were stained with anti-BrdU antibody (1 100; BD Biosciences, Franklin Lakes, NJ, USA) overnight at 4°C.

After washing twice with staining buffer, cells were stained with directly labelled anti-CD3 for 30 min on ice, washed and then fixed with 1% paraformaldehyde.

After the final rinse with the non-stringent wash buffer, the microarrays were stained with 1× Stain buffer (100 mM MES, 1 M Na+, 0.05% Tween20, 50 mg/ml of BSA, and 1 mg/ml of Cy3-streptavidin) at RT for 25 min. The stain buffer was removed and the microarrays were rinsed once more with non-stringent wash buffer.