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Immediately after treatments, all samples were disrupted in 350 μl of lysis buffer and were snap-frozen in liquid nitrogen.
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All tumors were ≤5 cm in diameter and were snap-frozen and stored at −80°C.
Tissues and fluids were snap-frozen and stored at −80 °C.
At the end of study, tumours were excised, weighed and about 30 mg were snap-frozen and homogenized in lysis buffer.
For immunohistochemistry, brains were fixed in 10% formalin neutral buffer solution (Wako), and for biochemical analysis, brains were snap-frozen on dry ice and stored at −80°C.
Tumour and matched normal samples were snap-frozen and stored at − 80 °C until DNA preparation.
Tumour and normal tissue samples were snap-frozen and stored at −80°C.
After 14 days, animals were fasted for 5 h, sacrificed, and tissue samples were snap-frozen.
Small tissue blocks were snap-frozen and stored at -80°C.
Globes were snap-frozen and stored at -80°C until use.
Samples were snap-frozen and stored at −70°C.
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CEO of Professional Science Editing for Scientists @ prosciediting.com