Sentence examples for buffer and were separated from inspiring English sources

The bound proteins were eluted with SDS loading buffer and were separated by SDS/PAGE followed by immunoblotting with antibody against STAT3 (Santa Cruz Biotechnology, Dallas, TX, USA).

Cell lysates were boiled in Laemmli sample buffer and were separated by SDS-PAGE, transferred to nitrocellulose filters, probed with the antibody of interest, and developed using ECL (Amersham Biosciences, Pittsburg, PA, USA).

Then, samples were reduced with β-mercaptoethanol in Laemmli sample buffer and were separated by SDS-PAGE electrophoresis.

Proteins bound to the beads were eluted with 2 × SDS-PAGE sample buffer and were separated on an SDS-PAGE gel.

The samples of surnatants were thereafter diluted with reducing sample buffer and were separated by electrophoresis on a 10% SDS-PAGE gel (20 μg protein per lane loaded).

The retained peptides were washed with the same buffer and were separated on a PicoFrit Analytical Columns (ProteoPep II C18, 300 Å, 5  μm, 50  μm × 100 mm, tip ID=10  μm, New Objective, Inc).

Eluates were vacuum dried and resuspended in protein sample buffer (1×MES buffer+1×protein loading buffer, Life Technologies) and were separated by SDS-PAGE on a 10 20% NuPAGE gradient gel (Life Technologies).

A total of 20 μg protein was boiled in the presence of 6 × sodium dodecyl sulfate sample buffer, and was separated on 7.5% or 10% sodium dodecyl sulfate-polyacrylamide gel (ATTO, Tokyo, Japan).

Briefly, human ADSC samples and epithelial-like cells were selected using the same method, lysed in RIPA buffer and protein lysates and were separated on 8 % SDS polyacryamide gel by electrophoresis.

The beads were washed three times with lysis buffer, and immunoprecipitates were separated from the beads by adding 2× sample buffer and boiled and fractioned by SDS PAGE.

The beads were washed three times with lysis buffer and immunoprecipitates were separated from the beads by adding 2× sample buffer and boiled.