Sentence examples for buffer and were resuspended from inspiring English sources

The pellets were then washed twice with HEPES buffer and were resuspended in 100 µl of the same buffer.

The enriched IE were washed twice with panning buffer and were resuspended in 10 ml of panning buffer at a concentration of approximately 5×107 IE/ml.

Then cells were washed with the FACS buffer and were resuspended in each tube with 500 μl of FACS buffer for FACS analysis.

The cells were washed with modified Tanaka buffer and were resuspended in the same buffer containing 5 μM of DAPI and 1 mM 2,4-dinitrophenol (DNP), and incubated at 37°C for 10 h [ 27, 28].

The pellet was washed with 50 mM Tris HCl buffer (pH 8.0) and was resuspended in 10 ml of the same buffer.

Primary tissues from patients with CRC, HCT116 and DLD-1 cells were treated with lysis RIPA buffer and proteins were resuspended in sample buffer and separated on 10% Tris-glycine gel using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

Cells were lysed in NP-40 lysis buffer, and nuclei were resuspended in MNase digestion buffer.

The supernatants were removed and replaced with fresh buffer, and NPs were resuspended by vigorous pipetting.

Erythrocytes were lysed using NH4Cl buffer, and cells were resuspended in media.

Membranes were washed once with 0.5 ml of swell buffer and finally were resuspended in 200 μl of FRB (10 mM Tris [pH 7.5], 1.5 mM KCl, 3 mM MgCl2, 1 mM EGTA, 0.32 M sucrose).

Cells were then washed by incubation buffer and after centrifugation were resuspended and incubated in fluorochrome-conjugated secondary antibody (polyclonal swine anti-rabbit IgG labeled with FITC 1 : 10, Dako Cytomation).