Sentence examples for buffer and were lysed from inspiring English sources

Post-efflux, cells were washed twice with quench buffer and were lysed for analysis of Fe (CPM) and protein content.

Following the indicated treatments, cells were washed by cold 1 X PBS buffer and were lysed using NP-40 cell lysis buffer (Life Technology).

Harvested cells (0.3 g, stationary phase) were suspended in 10 mM Tris HCl, pH 7.5 (buffer B), 100 mM NaCl (1 mL), and were lysed in buffer B, containing 1 % Nonidet P-40, 2 mM spermindine-HCl, 10 mM Na2EDTA, a cocktail of protease inhibitors (2 μg/mL), 1 mM PMSF (final volume 1 mL), and incubated at 10 °C for 30 min.

After 1 day, the cells were incubated with 500 μL Hank's balanced salt solution (HBSS) containing 0.5% bovine serum albumin (bHBSS), 3.7 kBq carrier-free 18F-TFB, and 10 μmol/L sodium iodide (specific activity 740 MBq/mmol) at 37 °C for 30 min. After incubation, the cells were washed twice with ice-cold bHBSS buffer as quickly as possible and were lysed using 500 μL 2% SDS.

Rat and mouse kidneys were placed in ice cold 0.1 M potassium phosphate, pH 7.4 buffer containing 1 mM 2-mercaptoethanol and were lysed by Polytron.

The frozen pellet was resuspended in 1x PBS (pH 7.4) at a pellet to buffer ration of 1∶10 (w/v) and was lysed by microfluidization (Model M110Y, Microfluidics).

Platelets were lysed in NP40 lysis buffer and megakaryocytes were lysed in RIPA buffer.

The reaction was stopped by washing cells twice with 1 mL of ice-cold wash buffer, and cells were lysed in 1 mL of 0.2 M NaOH.

Cells (1 × 10 cells/50  μL lysis buffer) were lysed in buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 5 mM EDTA, Protein Inhibitor Cocktail (ROCHE) and 1% Triton X-100 for 60 min at 4°C.

The recombinant plasmid pHR2 was isolated from L. reuteri HR2 using the plasmid miniprep kit, with the following modifications: The cells in resuspension buffer, were lysed with 30 mg/mL lysozyme (USB) and incubated at 37°C for 30 minutes.

60 mm culture plates were rinsed with phosphate buffer saline (PBS) and cells were lysed using 150 to 175 μl of lysis buffer (50 mM HEPES (pH 7.6), 250 mM NaCl, 0.5% Nonidet P-40, 0.5% Triton X-100, 5 mM EDTA, 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM Na2VO3 and 2 μg/ml each of aprotinin, bestatin, and leupeptin), incubating at 4°C for 30 minutes.