Buffers and samples were injected by a nonpulsatile piston pump into the 30 μL flow cell, which was mounted on the coupling prim.
To generate concentration gradients in the microfluidic device, the methanol and buffer solutions were injected with a uniform flow rate (66 μl/min) using a syringe pump (Harvard Apparatus, USA).
All analyzed samples and running buffer were injected to an rhEGFR-coated flow cell and uncoated reference cell.
Single bolus injections of 2.5 μg, 25 μg, and 250 μg rhBMP-7 (mammalian cell derived, CYT-276, ProSpec-Tany TechnoGene Ltd, Ness-ZIsraelIsrand), and a sucrose buffer (sham) were injected into the NPs in the T13-S1 segmentsegments in a balanced Latin square design.
elba1-3 and insv dsRNAs or buffer alone were injected into embryos transgenic for the ftz -4×pHS1-LacZ enhancereporterng reporter and blocking activity examined.
Different concentrations of Pikp-HMA (ranging from 1 to 1200 nM) and buffer only controls were injected over flow cells 1 and 2 (flow cell 1 was used as reference) for 120 s and dissociation was recorded for another 300 s.
After allowing the flowcell surfaces to equilibrate with HBS-EP running buffer, solutions of varying concentrations of TGFβ1, diluted in HBS-EP buffer, were injected using kinetic analysis injection protocols through Biacontrol software.
The stimulant (epinephrine, 10 μM) and buffer (culture medium) were injected at 1 μL/min into the channel via a pump.
MOs, diluted in Danieau buffer [16] were injected at the 1- to 2-cell stage.
The protein as well as the buffer samples were injected automatically using the sample-changing robot for solution scattering experiments at the SAXS station X33 [ 27].
