Sentence examples for buffer and were incubated from inspiring English sources

Briefly, slides were equilibrated with equilibration buffer and were incubated for 30 min at 37 °C with recombinant terminal deoxynucleotidyl transferase (rTdT) incubation buffer.

Membranes were blocked for 45 min at room temperature with 5% skim milk in 1 × TBST buffer and were incubated with the appropriate primary and secondary antibodies at 4 °C overnight or at room temperature for 1 h.

Membranes were washed twice, five minutes each in 1× Tropix assay buffer, and were incubated with CDP-Star for 5 minutes.

Soluble extracts from HEK 293T cells transfected with A2ML1-FLAG-His8 were prepared as described above using a pH 8 buffer and were incubated with Nickel-resin (Sigma-Aldricht) 1 h on ice, and the resin was subsequently submitted to extensive washing with Tris-HCl 50 mM, pH 8, NaCl 200 mM, NP40 1%, protease inhibitor.

Membranes were washed three times with blocking buffer, and were incubated with alkaline phosphatase-conjugated secondary antibodies for 30 minutes.

Supernatant was removed and samples were lysed with the addition of cold lysis buffer and were incubated on ice for 10 min.

Wires were separately dipped into polypropylene tubes containing 50 ml of buffer solution and were incubated and maintained at 37 °C.

Wires were separately dipped into polypropylene tubes containing 50 ml of buffer solution and were incubated at 37 °C and placed on a shaker to simulate in vivo situation.

Cells were washed with Annexin V binding buffer (Invitrogen), and were incubated with FITC-conjugated Annexin V for 30 min at room temperature.

Overall, 275 MBq of high purity 111In-chloride (specific activity >185 GBq/ μg indium) in diluted hydrochloric acid (Covidien, Petten, The Netherlands) or 1665 MBq of 90Y-chloride (IBA-Cis bio, Saclay, France) were added to 2.25 mg of OTSA101-DTPA in the presence of acetate buffer and was incubated 90 min at 37 °C.

The digestion mixture contained 1 unit of SUMO protease per 100 µg of the fusion protein in a volume of 500 µl of 1×PBS buffer and was incubated for 2 h at 30°C.