Cells were washed twice with cold PBS (phosphate buffer saline) and were harvested by trypsinization.
After three washes in PBS buffer, cells were harvested mechanically in RIPA buffer, and counts were measured with a gamma counter.
After washing the cells twice with binding buffer, they were harvested with a cotton stick and the cell-associated activity was measured in a gamma-counter.
The resulting supernatants in Buffer C were harvested as nuclear extracts.
Thymus and spleen were harvested into FACS buffer (PBS containing 2% FBS and 0.2% NaN3) and kept on ice.
Thirty-six hours after transfection, 15 μM MG132 was added to block proteasome degradation, and cells were harvested in EBC buffer containing protease inhibitors.
In order to study sex differences in the innate immune response, five male and female BALB/c mice were infected intraperitoneally with coxsackievirus B3 (CVB3) or phosphate buffered saline and their spleens were harvested 12 h later for microarray analysis.
Non-stressed, wounded (buffer inoculated) and RYMV inoculated leaves were harvested 2, 5 and 7 days post inoculation (dpi) for IR64 leaves and 3, 5, 7 dpi for Azucena leaves.
Adipose and muscle proteins were harvested in lysis buffer containing protease and phosphatase inhibitors and subjected to western blot analysis, as described [ 8].
Following experimental treatment culture supernatants were isolated and the cells were harvested in lysis buffer.
