Sentence examples for buffer and were boiled from inspiring English sources

After collecting unbound lysate, the beads were washed four times with the RIPA buffer, and were boiled in 2× SDS buffer.

Phosphotransferase reactions were stopped by the addition of 4X sample buffer and were boiled for 5 min before their fractionation through 10% SDS-PAGE.

Protein A beads were washed 3 times with lysis buffer (500 μL), suspended in Laemmli sample buffer (15 μL), and were boiled for 15 min to dissociate proteins from beads.

For western blotting, cultured cells were lysed directly in Laemmli buffer with dithiothreitol and were boiled.

Purified Omp29 and bacterial samples were dissolved in SDS-PAGE loading buffer (Invitrogen) containing 2-mercaptoethanol and were boiled for 10 min. Proteins separated in 4 12% gradient SDS polyacrylamide gel (Invitrogen) were trans-blotted onto a nitrocellulose (NC) paper.

The beads were washed with HB for 3 × 5 min. Proteins were eluted from the beads by addition of an equal volume of 4× Laemmli sample buffer (15) and being boiled for 10 min, separated by SDS−PAGE using a 10% acrylamide gel, and visualized by Coomassie Brilliant Blue staining.

An aliquot of 20-30 μg of total protein per lane was dissolved in loading buffer containing 2-mercaptoethanol and was boiled for 5 min.

Supernatants were incubated with the antibody-coupled Dynabeads for 60 min at 4°C, washed with RIPA buffer, and dynabeads were boiled in 20 µl of sample buffer and analyzed by Western blot.

Beads were washed several times with 1 ml IP buffer and proteins were boiled off Sepharose beads in Laemmli buffer containing 8M urea for SDS-Page.

The reaction was terminated by adding half of the reaction volume of three times concentrated Laemmli SDS sample buffer, and samples were boiled for 5 min. Phosphorylated GroES was resolved using 18% SDS PAGE, stained with CBB R-250 in 40% (v/v) methanol with 10% (v/v) acetic acid, dried, and autoradiographed as described above.

Samples were then incubated for 2(h at room temperature followed by extensive washing. After adding SDS-PAGE sample buffer, samples were boiled and centrifuged (10(min, 16 000(g), and the supernatant subjected to SDS-PAGE.