Sentence examples for buffer and were analyzed from inspiring English sources
Proteins were eluted in Laemmli sample buffer and were analyzed by SDS-PAGE followed by immunoblot with indicated antibodies.
Cells were washed twice in Staining Buffer and were analyzed using LSR 1 or LSRII (BD Bioscience) in the Stanford Shared FACS Facility.
The lysates were prepared from dissected sensory epithelia using hypotonic buffer and were analyzed by Western blotting either before or after endoglycosidaseF treatment (Roche, Indianapolis, IN).
Samples were washed in BSA buffer and were analyzed on a FACS Calibur.
After washing cells were resuspended in 400 μl of FACS buffer and were analyzed using a LSRII FACS machine (BD Bioscience, Heidelberg, Germany).
Following rigorous washing steps, samples were resuspended in 0.5 ml of FACS buffer and were analyzed using a LSRII FACS machine (BD Bioscience, Heidelberg, Germany).
The cells were washed in PhosFlow Perm/Wash Buffer I, and were analyzed by flow cytometry, as already described.
The reaction was stopped by adding the SDS-PAGE loading buffer and was analyzed by western blot and autoradiography.
The representative samples (5.0 g each) were extracted using the acetate buffered extraction under the optimized conditions and were analyzed using HPLC system of the reported methods.
Bead-coupled proteins were washed three times in binding buffer containing 1 M KCl and boiled in Laemmli buffer, and supernatants were analyzed by western blot analysis with α-RSK2 antibodies (Santa Cruz Biotechnology).
