Sentence examples for buffer and were added from inspiring English sources

Serum samples or controls were diluted in sample buffer and were added to coated microtiter wells.

The primary antibodies were diluted in the same blocking buffer and were added to detect the target proteins.

Primary antibody was diluted 1 500 in blocking buffer and was added and incubated overnight at 4 °C.

Primary antibody was diluted in blocking buffer and was added as follows: HSP27, 1 200; HSP60, 1 300; and Prx-2, 1 200.

The primary antibody was diluted 1 1000 in blocking buffer and was added to the membrane for 2 h.

Reducing buffer and sample buffer were added and sample was boiled at 70°C for 10 min.

40 μl proteinase K and Buffer BM2 were added and incubation continued for 10 min at 55°C.

The substrate, crude enzyme and buffer were added to tubes and incubated at 37 °C for 30 min.

The substrate crude enzyme and buffer were added to tubes similar to the amylase assay.

Specific substrate and detection buffer were added and the luminescence was measured.

Next, primers and PCR buffer were added and a PCR was performed.