Sentence examples for buffer and washes were from inspiring English sources

Primary and secondary antibody incubation was carried out in blocking buffer and washes were performed using KCM+T.

For the peach microarray the buffer and washes were from the Pronto!™ Universal Microarray Kit (Corning, NY, USA) and performed in accordance to the manufacturer's protocol.

Hybridisation buffer, incubation temperatures, removal of coverslips, and washes were done as described above for formalin fixed fungi.

All the solutions including cell lysis buffer, blocking buffer and wash buffer were from this kit and the experiment was performed following the manufacturer's instructions.

All buffers (protease buffer, lysis buffer and washing buffer) were pre-warmed before use and all experiments were performed in triplicate.

Beads were washed with small amount of lysis buffer, and the first two washes were pooled with the eluate.

Then, 5×105 cells were incubated with crude cell extract containing E2 proteins or control cell extract for 1 h at room temperature in washing buffer and were washed twice with PBS.

Before each run, the capillary system was conditioned by washing with the running buffer for 2 min. Buffers and washing solutions were prepared with Milli-Q water and filtered throughout 0.45-μm filters.

All buffers and wash conditions were performed using NimbleGen methods and protocols (www.nimblegen.com).

Animals were washed from plates using M9 buffer and were washed using centrifugation.

Inserts were fixed in 3% glutaraldehyde in 0.1 M sodium cacodylate buffer and were washed in 0.2 M sodium cacodylate buffer.