Sentence examples for buffer and was used from inspiring English sources

The pellet was washed several times with digitonin-free sucrose buffer and was used as the organelle fraction which was solubilized in RIPA buffer.

The C232S substitution obviates the need for reducing agent in the assay buffer and was used as the control for in vitro experiments (ATC232S).

After three times wash of the column, the His-KIAA0101 fusion protein was obtained by the elution buffer and was used for rabbit polyclonal antibody production according to the standard procedure.

The C232S substitution obviates the need for a reducing agent in the assay buffer and was used as the control for in vitro experiments; this variant has been found to behave in an equivalent fashion to wild-type protein in previous studies (for example [ 16, 36]).

The granules were resuspended in 100 µl of HEPES buffer and were used for the various microscopy and spectroscopy analyses.

Sections were encircled with rubber cement to enclose hybridization buffer and were used immediately in hybridization.

All cell extracts were adjusted to the same protein concentration by adding lysis buffer and were used for enzyme assays.

Specific control groups included (a) protein only, (b) peptide only, and (c) buffer only, and were used for background correction.

Two wash buffers (PBS and PBST) were tested to remove matrix compounds and PBST was found most suitable as wash buffer and the same buffer was used to resuspend the beads.

The protein concentration for SEC analysis was 30 µmol/L (~1 mg/mL) and buffer A was used as the elution buffer.

The TB medium without glycerol and PBS buffer was used as the control.