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The heat of dilution of NPLC0393 was measured by titrating NPLC0393 into the ITC buffer and was subtracted for data analysis.
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The dilution heat of the GlcNAc was measured by injecting GlcNAc solution into buffer alone and was subtracted from the experimental curves before data analysis.
Buffer was the baseline and was subtracted from the protein spectrum.
The heat of dilution was determined by adding the titrant to the corresponding buffer in the absence of protein and was subtracted to obtain the effective heat of binding.
A blank reaction containing only buffer and substrate was subtracted from the measurements obtained for each assay.
For data analysis, all diffraction images were azimuthally integrated, averaged and the buffer signal was subtracted from that of the buffered protein solution.
Twelve scans were averaged, and the buffer baseline was subtracted.
The scans were averaged, and the buffer spectrum was subtracted.
Four scans were averaged, and the buffer baseline was subtracted.
Three sweeps of the 180 260 nm wavelength range in 1.0 nm increments were averaged, and the buffer signal was subtracted from the protein signal before converting the raw data into residual molar ellipticity.
The resulting scattering curves for each sample were radially averaged, and the buffer was subtracted.
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