Sentence examples for buffer and was subjected from inspiring English sources
Labelled CD44 was released from the beads via incubation (100°C/5 minutes) in 2× reducing Lamelli sample buffer, and was subjected to SDS-PAGE and immunoblotting.
Samples were centrifuged (13 000 rpm for 5 min) and the lysate collected; pelleted material was resuspended in a second aliquot of lysis buffer and was subjected to the 15 min of vortexing followed by centrifugation.
Each sequencing reaction contained 5 μl total volume and contained 0.16 μM primer, 0.125 μl BigDye® Terminator v3.1 (Applied Biosystems), 0.875 × BigDye® Sequencing Buffer (Applied Biosystems) and was subjected to cycling conditions as described in the BigDye® v.3.1 protocol.
Finally, proteins were extracted from the beads with SDS sample buffer and were subjected to SDS acrylamide gel electrophoresis.
Beads were washed and boiled with SDS gel loading buffer and were subjected to western blot using anti HIPPI and anti P53 antibodies.
After incubation for 2 hours at 4°C, the beads were washed three times with the lysis buffer, and the immunoprecipitates were eluted using 3× Laemmli sample buffer and were subjected to SDS-PAGE and western blotting using indicated antibodies.
Retinas from WT mice (C57BL/6), rd7/rd7 Tg/Tg mice treated with AP20187, and rd7/rd7 Tg/Tg mice treated with vehicle were extracted using lysis buffer and were subjected to quantitative real-time PCR (qRT-PCR; n=3 in each group).
Immunoprecipitates were collected on Protein G agarose beads by overnight rotation, washed four times with lysis buffer, re-suspended in 2× Laemmli sample buffer, and were subjected to SDS-PAGE followed by Western blot analysis.
Reactions were terminated by the addition of 5 μl of SDS sample loading buffer and were subjected to SDS/PAGE (4 12% gel) analysis in the absence of any reducing agent.
