Sentence examples for buffer and was sonicated from inspiring English sources

Solution of 1.5 mg FMNPs-COOH was dispersed in 2 mL pH 7 PBS buffer and was sonicated for 10 min. Then we mixed 1 mL of fresh 400 mM EDC and 100 mM NHSS in pH 6.0 MES buffer and rotated it at room temperature for 15 min. After this, the resulting solution was separated by magnetic field, and 1 mg/mL of HAI-178 monoclonal antibody was added to the above mixture and stirred in dark place for 2 h.

The collection was dissolved with 1 × PBS buffer and was sonicated on ice in short bursts.

Nuclei were further purified by centrifugation through 0.88M sucrose and resuspended in 0.34M sucrose plus 0.5 mM magnesium acetate (sonication buffer), and were sonicated to release the nucleoli.

To determine the changes in the expression of Bcl-2 family proteins in TM40D cells, cells induced under different procedures were collected and homogenized in lysis buffer and were sonicated for 4 times for 5 seconds each on ice.

Equal amounts of protein in sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) sample buffer was sonicated and subjected to electrophoresis on 12.5% SDS-PAGE.

For control experiments in the absence of H2O2 and oxygen, 100 mL of the buffer was sonicated for 60 min, and residual oxygen was removed by purging with nitrogen.

The blood mixed with buffer was sonicated for 3 min, and its fluorescence measured with a fluorescence microplate reader (Tecan Japan Co., Ltd).

Nuclei were lysed using nucleus lysis buffer, and digested chromatin was sonicated to get equal sizes of chromatin fragments (average size about 500 base pairs).

Cell paste in start buffer was sonicated on ice for 20 min. After centrifugation (23700 g for 30 min, Beckman JA-20 rotor), the supernatant was filtered through a 0.45 μm syringe filter.

For immunoblot analysis, 10 cells were lysed in 100  μL of Laemmli lysis buffer and lysates were sonicated and separated by SDS-PAGE.

Supernatants were collected as the cytoplasmic fraction, while the pellet was washed in 200 μL of lysis buffer twice and the pellet was sonicated in 40 μL of Laemlli buffer as the nuclear fraction.