Total protein lysates were prepared using RIPA buffer with protease inhibitors (Roche, Mannheim, Germany) and were quantified using the Bio-Rad DC Protein Assay (Bio-Rad, Hercules, California, USA).
Cells were harvested in RIPA lysis buffer, and protein was quantified with the BCA assay (Beyotime).
For instance, the impact of conservation tillage and intercropping buffer strips was quantified in two watersheds in central Iowa in the USA [24].
The segregation ability of 3-[ 3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS), a zwitterionic surfactant, on cytochrome c (cyt c) aggregates in a phosphate buffer solution was quantified through the dynamic light-scattering analysis, and CHAPS was found to have an excellent ability in reducing nonspecific affinity among cyt c molecules.
The zinc concentration in each of the starting and final solutions, as well as in the 3 buffer washes, was quantified using UV/Vis spectroscopy in conjunction with a colorimetric zinc indicator (PV).
The plasmid was eluted with 200 μL of elution buffer and the yield was quantified by UV absorption.
The pellet was then eluted in 35 μl of TE buffer and total DNA was quantified by Quant-iT™ dsDNA BR kit (Invitrogen, Carlsbad, California).
Total protein was extracted from ovarian cancer cells using RIPA buffer and the protein was quantified with the BCA Assay Kit Thermo Scientificc, Rockford, IL, USA).
Tissue was harvested in Nonidet P-40 lysis buffer, and protein content was quantified according to the manufacturer's instructions (Bio-Rad Laboratories, Hempsteadpstead, UK).
Whole cell lysates of HepG2 cells treated with siRNA or ActD were prepared in RIPA buffer, and protein concentration was quantified using the BCA assay.
