Sentence examples for buffer and was incubated from inspiring English sources

Overall, 275 MBq of high purity 111In-chloride (specific activity >185 GBq/ μg indium) in diluted hydrochloric acid (Covidien, Petten, The Netherlands) or 1665 MBq of 90Y-chloride (IBA-Cis bio, Saclay, France) were added to 2.25 mg of OTSA101-DTPA in the presence of acetate buffer and was incubated 90 min at 37 °C.

The digestion mixture contained 1 unit of SUMO protease per 100 µg of the fusion protein in a volume of 500 µl of 1×PBS buffer and was incubated for 2 h at 30°C.

The digestion mixture contained 2 units of SUMO protease 1 per 100 µg of the fusion protein in a volume of 500 µl of 1×PBS buffer and was incubated for 3 h at 30°C prior to be adjusted to a pH value between 3.0 and 4.0.

Supernatant was diluted 1 50 with Tyrode buffer and was incubated for 30 minutes at room temperature in the dark with fluorochrome-labelled antibodies.

Phosphorylated serine 10 (phospho ser10) histone H3 was detected using a similar method, except blocking buffer contained 1.5% goat serum (Santa Cruz Biotechnology, Santa Cruz, CA), 1% bovine serum albumin, and primary antibody (catalog no. 06-570; Upstate CeLake Placidg SolutioNY, Lake Placidiluteddiluted 1 500 in blocking buffer and was incubated on slides for 1 hr.

Briefly, slides were equilibrated with equilibration buffer and were incubated for 30 min at 37 °C with recombinant terminal deoxynucleotidyl transferase (rTdT) incubation buffer.

Membranes were blocked for 45 min at room temperature with 5% skim milk in 1 × TBST buffer and were incubated with the appropriate primary and secondary antibodies at 4 °C overnight or at room temperature for 1 h.

Membranes were washed twice, five minutes each in 1× Tropix assay buffer, and were incubated with CDP-Star for 5 minutes.

Soluble extracts from HEK 293T cells transfected with A2ML1-FLAG-His8 were prepared as described above using a pH 8 buffer and were incubated with Nickel-resin (Sigma-Aldricht) 1 h on ice, and the resin was subsequently submitted to extensive washing with Tris-HCl 50 mM, pH 8, NaCl 200 mM, NP40 1%, protease inhibitor.

Membranes were washed three times with blocking buffer, and were incubated with alkaline phosphatase-conjugated secondary antibodies for 30 minutes.

After transfer, the membranes were saturated with blocking buffer and were incubated with rabbit immunoaffinity purified IgG anti-citrulline (Upstate Biotechnology, Lake Placid, NY, USA).