Sentence examples for buffer and was allowed from inspiring English sources

The supernatant was added to a Ni2+-NTA resin column (1 ml bed volume) equilibrated with lysis buffer and was allowed to bind slowly.

Then, 90 μL of the probe-DNA in DTT solution was added, followed by 410 μL of the DNA attachment buffer, and was allowed to enter the gel bed completely.

The swollen sensor was mounted in the QCM-D flow cell filled with the same buffer and was allowed to swell again until no appreciable frequency shifts were observed.

The resultant F-18-SFB was coupled with [R8,15,21, L]-VIP through the process briefly described next: about 1850 MBq (50 mCi) F-18-SFB was dissolved in 50  μL acetonitrile, to which was added 100  μg [R8,15,21, L]-VIP (in 200  μL 0.1 M borax-boric acid buffer), and was allowed to react under room temperature for 20 min.

The pH was not buffered and was allowed to change as a result of solution-metal/alloy interactions and was recorded before and after the experiment.

Antigen retrieval was accomplished using 0.01M preheated citrate buffer (pH −6.0, °C °C) for 15 min and was allowed to cool at room temperature.

The column was connected to the mass spectrometer and syringe pumps (OEM PSD/3 Syringe Pump, Hamilton Company) and was allowed to equilibrate with buffer C until the background level of the ion [M+H]+ signal was stable.

Homogenates were transferred with an additional 1 mL of buffer to microcentrifuge tubes and were allowed to precipitate overnight at -20°C.

Fluorescent-labeled (Amersham, RPN 3540) fragments (500 Ηg) of thevitellogeninclones AP4a5 or AmR9 (MD Piulachs, J Cruz, X Bellés, KRG, AR Barchuk and ZLPS, submitted) were added in the prehybridization buffer and hybridization was allowed to proceed over night.

Mcllvaine buffer solution was allowed to slowly pass though the coal bed in 15 minutes.

This Ni resin/wash buffer mixture was allowed to incubate on a rotator for an additional 15 min, at which point the resin was pelleted and the wash buffer was decanted.