Sentence examples for buffer and was added from inspiring English sources
Primary antibody was diluted 1 500 in blocking buffer and was added and incubated overnight at 4 °C.
The primary antibody was diluted 1 1000 in blocking buffer and was added to the membrane for 2 h.
Primary antibody was diluted in blocking buffer and was added as follows: HSP27, 1 200; HSP60, 1 300; and Prx-2, 1 200.
CD31 enzyme-linked immunosorbent assay (ELISA) substrate was prepared by dissolving p-nitrophenol phosphate in Tris buffer and was added in accordance with the manufacturer's instructions.
The tissue culture dish was quickly frozen with liquid nitrogen, which was allowed to warm up to room temperature and 1 ml lysis buffer and was added with PMSF protease inhibitor (Thermo scientific and Sigma-Aldrich Co).
The primary antibodies were diluted in the same blocking buffer and were added to detect the target proteins.
Upon 5 washes with lysis buffer sample buffer was added and samples were subjected to SDS-PAGE and analysed upon blotting using tag-specific antibodies (Sigma).
Immunoprecipitates were washed three times with lysis buffer, and sample buffer was added to 1X final, and samples were boiled at 95°C for 5 minutes.
The samples were diluted eight-fold with buffer, and CaCl2 was added to 1 mM.
Washout was performed with a buffer and tetramethylbenzidine was added as a substrate.
