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DNA was solubilized in TE buffer and visualized on agarose gel stained by ethidium bromide.
The bound proteins were eluted with 0.3 M imidazole in equilibrating buffer and visualized on SDS/PAGE gels by Coomassie Blue staining.
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Finally, blots were washed for 3 × 5 min in washing buffer, developed using the enhanced chemiluminescence kit (ECLplus, GE Healthcare), and visualized on Kodak film.
After LPS binding, membranes were washed 3 times, 10 min each time in the above buffers and visualized for radioactivity on Bas 2000 radioimaging system (Fuji, Japan).
The insoluble fraction of the C. elegans proteome can be separated with strong detergent buffers such as SDS, where the remaining fraction (the 'insolublome') can be re-solubilized by formic acid and visualized on SDS-PAGE gels.
Supernatant and beads were separated and visualized on SDS-PAGE.
The cells were subsequently washed 2 times in annexin V binding buffer and then visualized on a Nikon inverted fluorescent microscope equipped with a digital camera.
The cover slips were mounted on microscope slides in the mounting buffer and visualized using a Zeiss Axio Imager z1 fluorescence microscope (Carl Zeiss Inc).
The amplified products were electrophoresed on 1.5% agarose gels in TAE buffer and visualized by ethidium bromide staining.
Digested PCR products were separated by electrophoresis on a 3% agarose gel in a TAE buffer and visualized by ethidium bromide staining under UV light.
PCR products were separated on 1% agarose gel in 1 × TAE buffer and visualized under UV light after staining with GelStain (Transgen, Beijing, China).
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