Sentence examples for buffer and used at from inspiring English sources
CpG were dissolved in Tris-EDTA buffer and used at a final concentration of 3 µM.
Bacteria from logarithmic phase cultures in TSB were washed twice with incubation buffer and used at a final concentration of 1×106 CFU/ml, based on optical density.
Dictyostelium cells growing in suspension were harvested, washed, resuspended in Soerensen phosphate buffer and used at a density of 1×105 cells/ml for inoculation of the E. coli B/r suspension.
Rnd3 C-terminal peptides were solubilized in ITC buffer and used at 500 600 μM.
Antibodies were diluted in this buffer and used at a final concentration of 25 μg ml−1.
The reaction products were precipitated and resuspended in 200 μl of hybridization buffer and used at the working dilution of 1 1,000.
Although previous work had shown that the antigen-antibody interactions are typically optimum at pH > 7 [ 24], fungal derived enzymes are typically buffered and used at around pH < 5. We therefore brought the enzyme samples up to pH 7.5 using PBS buffer prior to their addition to the wells.
The PAD inhibitor Cl-amidine [37] was dissolved in phosphate buffer saline (PBS) and used at different concentrations (0.1 500 μM final concentration).
Therefore, the pellets were suspended in Ni-NTA sonication buffer and used for detergent solubilization at 4°C for 1 h with 0.5% of detergents: Brij 35 (Aldrich Chemical Corp ., CHAPS (Sigma), NP-40 (Sigma), SDS (Fisher), Triton X-100 (International Biotechnologies Inc ., Tween 20 and Tween 80 (Sigma).
To investigate the stability of oxytocin in the presence of a combination of buffer and monovalent metal ions, citrate and acetate buffer were used at a concentration of 10 mM, in combination with the monovalent metal ions, sodium and potassium, added at a concentration of 10 and 20 mM (excluding sodium from the buffer component).
Secondary anti-rabbit-horseradish peroxidase conjugate (Pierce Biotechnology) in blocking buffer was used at 1 10000 for Gapdh, Smad3, and phospho- Smad3 for 1 h.
