Sentence examples for buffer and used as from inspiring English sources

The insoluble fraction was resuspended in the same volume of cytoplasmic buffer and used as the aggregate fraction.

The pellet was washed twice with mitoprep buffer and used as total membranes.

50 μL cell lysate was solved with SDS loading buffer and used as the input (10%), and the left was incubated with antibodies and protein G beads for 2 4 h at 4°C.

One portion was used as the whole sample; while the other portion was centrifuged, the supernatant was collected as the extracellular sample, and the cell pellets was resuspended in the same volumes of PBS buffer and used as the intracellular sample.

Pellets were re-suspended in equal volume of lysis buffer and used as nuclear fraction.

Lysates were brought to equivalent protein concentration and 30 µl of lysates were boiled with 30 µl of 2 times concentrated SDS-sample buffer and used as total extracts (TE).

Samples were diluted to 100 ng/μl in TE-buffer and used as templates for PorcineSNP60 Genotyping BeadChip (Illumina).

1% starch solution was prepared in 50 mM phosphate buffer (pH7) and used as substrate.

1% CMC solution was prepared in 50 mM phosphate buffer (pH7) and used as the substrate.

Supernatants were regarded as cytosolic fractions, pellets were washed with 0.4 ml buffer, centrifuged again at 20,800 ×  g and 4 °C for 10 min, resuspended in equal volumes of buffer as cytosols and used as mitochondrial fractions.

1% casein was prepared in 50 mM potassium phosphate buffer (pH 7.5) and used as substrate.