Sentence examples for buffer and treated for from inspiring English sources

Kinase reactions were terminated by addition of 15  μl 3 × SDS sample buffer and treated for 5 min at 100°C.

The nucleic acid pellet is resuspended in TE buffer and treated for 1 hr at 37°C with RNaseA, followed by incubation for 1 hr with 2 mg/ml proteinase K in the presence of 1% SDS at 60°C.

Five microliters of dried Sepharose protein-G (4 Fast-flow Sepharose, Amersham) were washed with 500 μl lysis buffer and treated for 1 h at 4 °C with 500 μl lysis buffer added with 5% BSA.

DNA precipitates were washed twice in 70% (v/v) ethanol, dissolved in TE buffer, and treated for 1 h at 37°C with RNase A. Finally, DNA preparations were electrophoresed in 1% agarose gel, stained with ethidium bromide and observed under UV light.

After thawing, filters were incubated for 20 min at 98 °C in an EDTA buffer (DAKO Target retrieval buffer, pH=9.9; Dako, Glostrup, Denmark), and treated for 5 min by triton X-100 at room temperature, in a humidity chamber for cell permeabilisation.

They were then microwaved in 10 mM citrate buffer, pH 6.0, to unmask epitopes and treated for 10 min with 1% hydrogen peroxide to block endogenous peroxidase.

Briefly, paraffin embedded tissue sections were deparaffinized and rehydrated and treated for antigen retrieval (0.01 M citrate buffer pH 6.0).

Following centrifugation, the pellet was resuspended in 200 µl of homogenization buffer and treated with PK for 1 h at 37°C (murine samples: 30 µg/ml; ovine and bovine samples: 75 µg/ml).

Cells were washed once, resuspended in sucrose buffer and treated or not for 15 min at 37°C with the AR blockers to test.

Sections were deparaffinised and rehydrated before undergoing antigen retrieval under pressure in 0.01  M sodium citrate buffer and were treated for endogenous peroxidase activity.

For the immunoaffinity purification of native NirS or NirN, 2 g of anaerobically grown P. aeruginosa PAO1 cells were resuspended in 10 ml of lysis buffer and treated as described above for the co-immunoprecipitation.