Liver tissue was stored in ice-cold Krebs–Henseleit buffer and transported to the laboratory within 30 min.
All sampled specimens from respective carcasses were pooled together and kept in phosphate buffer saline (pH 7.2) at 4°C in universal containers and transported on ice to the AHRI laboratory in Addis Ababa within one week.
The biosensor cells were trypsinized (Gibco), diluted in FACS buffer (95% (v/v) PBS, 5% (v/v) BCS, 1% (v/v) penicillin-streptomycin), and transported on ice to the Swanson Biotechnology Center Flow Cytometry Facility (Koch Institute, MIT).
A sample of liver tissue without distinction of lobes was excised using sterile scalpel blades and transferred immediately into ice cold sterile Krebs Ringers-4- 2-hydroxyethyl -1-piperazineethanesulfonic Ringers-4- 2-hydroxyethyl -1-piperazineethanesulfonicoratory Ringers-4- 2-hydroxyethyl -1-piperazineethanesulfonice bath.
A second swab tip was placed in a cryovial eluted in 0.9 ml phosphate buffered saline and stored at -80°C until transported on dry ice by express courier to the Oral Microbiology Laboratory at the University of Berne, Switzerland, for analysis of microbial content.
The excised tissue was immediately placed in modified KREBS-Henseleit buffer [ 38], placed on ice and transported from UCI Medical Center to UCI Main Campus.
On d 44, calves were killed and tissues from the rumen, omasum, duodenum, jejunum, ileum, cecum, and proximal and distal colon were collected, rinsed, and transported in buffer solution (pH 7.4 at 38.5°C).
This is most probably because of temperature differences, as the liver was transported on ice, sliced at low temperatures with cold buffers, and then incubated at 37 °C.
Specimens were transported on ice to the laboratory and the tissue was washed with ice-cold phosphate buffered saline (PBS), cut in small pieces, frozen in liquid nitrogen and stored at −80°C until use.
In short, aspirin buffers and transports the protons.
