Sentence examples for buffer and transfer from inspiring English sources

First in, first-out (FIFO) buffers were built into the FPGA boards to buffer and transfer data between boards without loss.

Reducing conditions included addition of anti-oxidant (Invitrogen) to running buffer and transfer buffer to prevent oxidation of reduced cysteine, methionine and tryptophan residues.

Polyvinylidene difluoride (PVDF) membranes, Tris-acetate mini gels (3 to 8%), LDS sample buffer, Tris-acetate SDS running buffer and transfer buffer were from Invitrogen (Carlsbad, CA, USA).

The cleavage reaction was started by adding substrate in fivefold molar excess over the enzyme, and let proceed for 30 min at 37°C, after which it was stopped by the addition of SDS-PAGE sample buffer and transfer on ice.

Agarose beads were washed four times with NP-40 buffer, and transferred onto a Micro-Spin Column (Thermo Fisher Scientific).

In copying, the recorder pulls data into a storage buffer and transfers it to the CD, maintaining a steady writing speed.

After a short spin, the cells were suspended in 300 μL of ice-cold PBS buffer and transferred to fresh tubes for FACS analysis.

Atomic force microscopy was routinely used to characterize the resulting PrP23-144 amyloid fibrils26, 36, which were subsequently washed with the 50 mM potassium phosphate pH 6.4 buffer and transferred by centrifugation to 3.2 mm solid-state NMR zirconia rotors36.

Cell lysates were heated to 95 100 °C for 5 min in sample buffer (187.5 mM Tris-HCl, 6% w/v SDS, 30% glycerol, 150 mM DTT, 0.03% w/v bromphenol blue), and an equal amount of protein was loaded on precast SDS-PAGE (Thermo Fisher Scientific) in running buffer and transferred to nitrocellulose membrane.

Cells were then suspended in staining buffer and transferred for flow cytometry (BD LSR II, BD biosciences).

L4 or young adults were washed three times with M9 buffer and transferred to the killing plates.