Sentence examples for buffer and titrated into from inspiring English sources

The foldamers were prepared at final concentrations of 1 mM in TBS buffer and titrated into TBS buffer in the calorimetric cell at 25 °C.

The Fyn SH3 domain peptide was dissolved in the same buffer and titrated into the xα2-peptide solution to observe the chemical shift changes of the xα2-peptide in 2D N-HSQC spectra at different molar ratios (1 0, 1 0.1, 1 0.2, 1 0.4, 1 0.5, 1 0.6, 1 0.7, 1 0.8, 1 1.0, 1 1.2, 1 1.2).

Circles correspond to the solute dissolved in the phosphate 0.1 M and pH 7.4 media and titrated into the same buffer while squares represent the solute dissolved in pure water and titrated into the same solvent.

In experiments aimed to measure the affinity of cPAH and its mutants for its native metal, Fe(II), 500 μM ferrous ammonium sulfate was prepared in dialysis buffer containing 2.5 mM tris(carboxyethyl phosphine (TCEP) and titrated into a 50 μM solution of protein also including 2.5 mM TCEP.

This pool was diluted and titrated into to 1 μg aliquots of COLO-829 total RNA (ATCC 1974).

After removal of liquid, the pellet was resuspended in SDS-PAGE sample buffer and titrated with Tris base if necessary.

A solution of highly concentrated AA, dissolved in buffer A, was titrated into a sample cell containing buffer A only.

Concentrated ligand solutions in Buffer B were titrated into a 400 µl sample of protein (180 nM in Buffer B) and Trp fluorescence monitored.

Concentrated hUNG in Buffer B was titrated into a cuvette containing 100 nM of fluorescein labeled DNA in Buffer B. After each addition, the solution was allowed to equilibrate for 4 min inside the fluorometer, and three measurements were averaged.

Buffer alone was titrated into protein sample to confirm that the heat of protein dilution was negligible.

The stock ligand solution (1000 µM) was diluted to a concentration of 10 µM with the buffer solution prior to ITC experiments and was titrated into the DNA solution.