Sentence examples for buffer and then were from inspiring English sources

Membranes were blocked for 45 min at room temperature with 5% skim milk in 1 × TBST (Tris-buffered saline and Tween 20) buffer, and then were incubated with the appropriate primary and secondary antibodies at 4 °C overnight and at room temperature for 1 h, respectively.

Leaves were washed twice with the loading buffer and then were observed with the Zeiss LSM 510 confocal microscopy system.

Next, a certain amount of monoclonal CRP antibodies were added into the QDs and dissolved in BS buffer and then were blocked by BSA.

The samples were washed with TBS buffer and then were counterstained with 4′,6′-diamidino-2-phenylindole (DAPI).

Briefly, the frozen and thawed samples were diluted in 1x dilution buffer and then were dropped onto the test card.

The tissues were washed twice in the phosphate buffer and then were put into GUS staining buffer containing 0.5 mg/ml 5-bromo-4-chloro-3-indolyl glucuronide.

The supernatant was taken out and mixed with the same volume of loading buffer and then was analyzed with SDS-PAGE.

Briefly, the 14N/15N mixture was added to 10 ml of buffer and then was centrifuged at 800× g for 15 minutes.

Pellet was washed with same buffer and then was resuspended in 20 mM Tris-HCl (pH8), 100 mM NaCl, 1 mM EGTA, 1 mM EDTA, 0.5% sodiumdeoxycholate, 0.1% SDS, 1% TritonX-100, containing protease inhibitors.

Agarose bead-DNA-protein complexes were washed three times with ice cold binding buffers and then were eluted in SDS-PAGE loading buffer by heating at 95°C.

Samples were washed with PBS 1X buffer twice and then were incubated with 2', 7'-dichlorofluorescein diacetate (H2DCFDA, for ROS detection) or propidium iodide (PI, to measure membrane damage) for 10 min.