Sentence examples for buffer and then washed from inspiring English sources

The protein-bound resin was loaded onto a column, washed with 20 ml of binding buffer and then washed sequentially using the binding buffer containing 20 and 40 mM imidazole.

The cells were washed once with Washing Buffer and then washed again by adding 1X Permeabilization Buffer; anti-human FOXP3 or Isotype Rat IgG2a control antibody (and intracellular antibodies when stained) was added and samples were incubated for 30 minutes in the dark (room temperature).

Blots were briefly rinsed with two changes of TBS-Tween buffer, and then washed three times for 10 min each.

These samples were washed three times with 500 μl of 1×cell lysis buffer, and then washed twice with 500 μl of 1×kinase buffer.

Samples were incubated with universal buffer and then washed with distilled water, applying suction to the filter plate either continuously or intermittently.

After embedding cells in 1% agarose the cells were lysed for 1 h in lysis buffer and then washed for 1 h in distilled water.

The immobilised template was treated for 5 s each with 70% ethanol, denaturation buffer, and then washing buffer, and transferred to a PSQ 96 plate (Biotage, Uppsala, Sweden).

Complexes were collected in a protein A agarose-salmon sperm DNA slurry for 1 hour at 4°C, washed once each with the provided low-salt, high-salt, and LiCl wash buffers, and then washed twice in Tris-EDTA buffer [10 mmol/L Tris-HCl (pH 8.0) and 1 mmol/L EDTA].

The hybridized microarrays were disassembled at room temperature in Gene Expression Wash Buffer 1 (5188–5325) and then washed in Gene Expression Wash Buffer 1 at room temperature for 1 min.

Whole embryos were incubated overnight at 4°C in a 1∶500 dilution of affinity-purified anti-Pacsin3 in buffer B and then washed extensively with several changes of buffer B over 2 3 h.

Afterwards, wells were blocked for 2 h by addition of 200 µl blocking buffer [protein free (TBS) blocking buffer (Perbio)] and then washed three times using 300 µl of wash buffer [protein free T20 (TBS) blocking buffer (Perbio)].